| Original document(7 pages) 中文版 |
The method to modify wild NPV and construct the new for Lepidoptera pest with anti-sense- genetic technique is presented and comprises the scheme as: (1) cloning CS conservative sequence of geometrid; (2) constructing the transfer carrier for the anti-sense CS gene of Lepidoptera pest; (3) recombining NPV; (4) validating the NPV with PCR or other methods; (5) contrast with wild NPV, evaluating the insect disinfectation capacity of recombined NPV for screening the virus strain with higher virulence and faster diffusion and secondary infection speed. |
Application Number
申请号 |
200510062132 |
Application Date
申请日 |
2005.12.21 |
| Title 名称 |
Method for reforming high-virulent nuclear polyhedrosis virus of lepidoptera pest
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Publication Number
公开号 |
1804031 |
Publication Date
公开日 |
2006.07.19 |
| Approval Pub. Date |
|
Granted Pub. Date |
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| International Classification 分类号 |
C12N7/01,C12N15/63,C12N15/66,C12Q1/68,A01N63/00 |
Applicant(s) Name
申请人 |
Zhejiang University |
| Address 地址 |
310027 |
| Inventor(s) Name 发明人 |
Lu Jianliang, Lin Chen, Liang Yuerong, Zhang Guanghui, Du Yingying |
| Attorney & Agent 代理人 |
feng ziling |
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