| Original document(6 pages) 中文版 |
The invention discloses a method for strawberry virus-removing, comprising the following steps: 1) putting the potted strawberry with virus into the bio-chemical incubator, culturing in the lethal temperature for virus for 2-3 days; 2) stripping the stem tip nascent tissue of smaller than 0.5mm, inoculating which in the culture medium with concentration of MS half-reduced for its growing; 3) transferring the stem tip nascent tissue when its sprout grows to 0.5-1.5cm in height to induced enrichment culture medium for growing; transferring it to biochemical incubator when generates adventitious bud, culturing under the lethal temperature for 30 minutes; 4) putting the sprout mentioned above into tissue culture room for culturing under 28 Deg C for 30 minutes; 5) repeating the steps 2) to 4) for 1-2 times. The method in this invention does not strip large quantity of stem tip and the virus-removing rate is as high as 100%. |
Application Number
申请号 |
200510062199 |
Application Date
申请日 |
2005.12.26 |
| Title 名称 |
Strawberry virus elimination method
|
Publication Number
公开号 |
1799341 |
Publication Date
公开日 |
2006.07.12 |
| Approval Pub. Date |
|
Granted Pub. Date |
|
| International Classification 分类号 |
A01H4/00,A01H3/00,C12N5/04,A01G7/00,A01G1/00 |
Applicant(s) Name
申请人 |
Zhejiang University |
| Address 地址 |
310027 |
| Inventor(s) Name 发明人 |
Mao Bizeng |
| Attorney & Agent 代理人 |
tang yinyi |
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