Process for reproducing virus of large brill hemorrhagic septicaemia by large brill fin clone

Original document(4 pages) 中文版

The invention relates to the method used turbot fin cell line to breed turbot haemorrhagic septicemia virus. It includes the following steps: adopting turbot fin cell line cells as breeding system; inoculating the virus to the cell line cells; adsorbing at 22 centigrade degree for 2h; adding into 10% calf serum MEM culture solution to culture; gaining culture after inoculated 4 days; collecting turbot haemorrhagic septicemia virus liquid after once freeze thawing at -30 centigrade degree. The bred virus has large amounts and strong infection capability, and can be directly applied to viral vaccine manufacturing and production to improve turbot survival rate.

Application Number 申请号	200510045184	Application Date 申请日	2005.11.17
Title 名称	Process for reproducing virus of large brill hemorrhagic septicaemia by large brill fin clone
Publication Number 公开号	1793342	Publication Date 公开日	2006.06.28
Approval Pub. Date		Granted Pub. Date
International Classification 分类号	C12N7/00,C12N7/02
Applicant(s) Name 申请人	China Oceanology Univ.
Address 地址	266003
Inventor(s) Name 发明人	Fan Tingjun, Cong Rishan, Wang Liyan, Geng Xiaofen, Yang Xiuxia, Li Mingyu, Yu Qiutao
Attorney & Agent 代理人	zhang zhongnan

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