The invention relates to a turbot fin cell line forming method. It includes the following steps: using pectoral fin and pelvic fin tissue as raw material; shearing the fin tissue adopting trypsin, hyaluronidase, II type collagenase multiple step digestion process to gain free fin cell and loosen tissue block; and culturing in L-15 culture solution containing 20% fetal calf serum, carboxymethyl chitin, N- acetyl glucose hydrochloride, glucosamine hydrochloride, human epidermic cell growth factor, human alkali fibroblast growth factor, and tooth bothid gill cell culture solution; adopting trypsin digestion process to do sub-culturing. The technology is scientific. The formed sub-culturing cell reaches the 62th generations. The formed turbot fin cell is not transfected by any virus or oncogene, and can hopefully be directly applied to correlation theory study and viral vaccine development. |