检测心肌肌钙蛋白 I,并建立两种检测方法 :直接法
的最低检测限为 2 .5 μg/L,基于传感膜上的夹心免疫
为 0 .5 μg/L,检测范围为 0 .5～ 2 0 μg/L,批内及批间精密度
分别为 3 .5 %～ 4.9% ,6.1 %～ 7.4% ;用夹心法及国外试剂
盒对 40名健康献血者和 2 0例急性心肌梗死患者血清
心肌肌钙蛋白 I水平进行检测 ,两者符合率为 95 % .
Cardiac and skeletal muscle troponin I(cTnI, sTnI) were purified from human left ventricular tissue and skeletal muscle tissue by affinity chromatographic method, respectively. The specific polyclonal antibody to cTnI was prepared by immuned pure New Zealand rabbit with the purified cTnI and purified by nitrocellulose membrance filtration-affinity chromatography with sTnI. A specific immunobiosensor for cTnI was developed by using surface plasmon resonance(SPR) biosensor with biotin as an intermediate layer...