情况的应用价值。 方法 利用Nested PCR
样品中鼠隐孢子虫卵囊进行检测。 结果 Nested PCR方法的检出
Objective To assess the value of 4 methods to detect Cryptosporidium spp in milk, faeces and polluted water. Methods Nested PCR, PCR, Sheather's floating and AP-AFS were used to detect Cryptosporidium spp in 119 samples respectively. Results Among 4 different methods, Nested PCR was the most sensitive one, by which, the positive rate of 11. 11% , 23. 29% and 0 were got in milk, feces and polluted water respectively. Conclusion Nested PCR could be applied in micro-detection of Cryptosporidium spp , for its h...