A method for the determining of Benzo (α) pyrene residue in tea by HPLC with fluorescence detection (HPLC-RF) was developed. Samples were purified by SPE, and analyzed by HPLC-RF. Within the linear range of 0.2 -50 μg/L, the linear equation was Y=3.85×105X+7.47×103( R=0.9991). The recovery tests of green and red teas ranged from 76.9% to 86.9%. The variation coefficients and detection limit were 2.0-4.0% and 0.2 μg/kg (S/N=3), respectively. This method was suitable for the qualitative and quantitative analy...