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nature : the use of special effects reversible reaction of macromolecular biochemistry selective isolation and purification of a liquid chromatography with the technology. Life phenomenon is the use of biological molecules exist between the very specific interactions, such as enzyme and substrate, and enzyme inhibitors, enzymes and coenzyme, and hormone receptor cells, and vitamin-binding protein, nucleic acid and groups, antibody and antigen affinity with each other and so will create reversible coordination compounds . Using Reversible Coordination Compounds of association and dissociation established principle of a simple, rapid, high recovery and purification of new technology. That can be measured is a macromolecular effects coordinating role in the coordination body (such as an enzyme or antigen) followed by the vector particles composed of stationary phase, the mobile phase is dissolved in a suitable buffer solution of the sample, which contains biological macromolecules (such as enzyme inhibitors, antibodies, etc.). When the sample solution through the column and then the same elution buffer solution, remove all impurities, and back to the other kind is not conducive to the interaction of enzyme inhibitors buffer solution, will inhibitor cleared out. The law can be separated from the body of trace elements. For pure enzymes, enzyme inhibitors, antibody, antigen, various combinations of proteins, hormones and the drug receptors, nucleic acid, group, multi-enzyme system. To study the biological and in vivo molecular interactions and mechanisms for effective means.
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